5. (2 pts) Look at the image below of an RNA molecule. Is it mRNA, RNA or tRNA? How can you tell? m?GPPP m7CDDD AAAAAAAA What is m7GPPP? PH]mRNA (cpm, thousands) You take this transcript and use an enzyme to remove the m7GPPP structure. You then incubate your mRNA with ribosomes, and the complexes were separated from free mRNA by sucrose density gradient centrifugation. Which one of these is likely to represent what you get? Location in tube (bottom) (top) [2]mRNA (cpm, thousands) 6. (1 pts) What would be the effect of a mutation in Sigma factor that binds it irreversibly to the RNA polymerase? Location in tube (bottom) (top)